UV spectrophotometric method development and validation for estimation of ketoconazole in bulk and pharmaceutical dosage form

Abstract

: For the determination of ketoconazole in bulk and pharmaceutical dosage form in accordance with ICH guidelines, a new, economical, precise, sensitive, linear, accurate, and quick UV-Spectrophotometric approach has been developed in Methylene chloride. The UV-visible spectrum of Ketoconazole was examined to determine its maximum absorption wavelength that is λ max at 255.2 nm. Linearity, accuracy, precision and robustness tested for this procedure.: Ketoconazole was found to have a maximum absorbance at 255.2 nm. The regression coefficient for the concentration range of 5–25 µg/ml. Ketoconazole. LOD and LOQ were determined to be 0.0225 and 0.75 µg/ml, respectively. The procedure was successfully used on ketoconazole in commercial formulation, and the outcomes were in good agreement with label claims.Depending on the results, the given method can be successfully applied of Ketoconazole in bulk and Pharmaceutical dosage form.