Development and validation of UV-visible spectrophotometric method for determination of Bicalutamide in bulk and in pharmaceutical dosage form

Abstract

The aim of the present study, simple and sensitive spectrophotometric method was developed for the estimation and validation of Bicalutamide in bulk and Pharmaceutical dosage form by UV spectroscopy as per ICH guidlines. The maximum absorbance (λmax) of Bicalutamide in methanol was found to be 272 nm against methanol as a blank. The Bicalutamide obeys the Beers law in the concentration range 2.5 to 15 μg/ml with a correlation coefficient of 0.999. The proposed method was applied for the estimation of Bicalutamide in pharmaceutical dosage form and % purity was found to be 99.96 to 100.06 that indicate the good agreement with the label claim. The accuracy of the proposed method was determined by recovery study at three different levels (50%, 100%, and 150%). The % recovery was found to be 99.6 to 100.2%. The precision study was carried out by intraday and interday. The % RSD was found to be < 2 which indicates that the method is precise. The proposed method was a fast technique for the routine analysis of Bicalutamide in bulk and pharmaceutical dosage form.