Utilizing In-vitro Techniques to Evaluate the Antioxidant Potential of Vitex negundo Leaves Extract

Abstract

In this study, Vitex negundo leaf extracts were screened for phytochemical composition and antioxidant activity. Antioxidants are essential for preventing and battling oxidative stress and, ultimately, ailments it leads to. The study that employed FRAP, nitric oxide scavenging, superoxide radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) to test the antioxidant activity of V. negundo leaves extract revealed antioxidant activity. The result of the phytochemical examination of the extract shows that entity of total phenolic content, reducing sugar, saponin. The findings suggest that this herb can be a very beneficial component for conditions that call for antioxidant therapy and are brought on by oxidative stress. The V. negundo shows the average TPC of 235.24 GAE/g weight of ethanolic extract. The TFC in the ethanolic extract was found to be 157.53 mg QE/g of extract. Outcomes demonstrated the significant antioxidant activity of ethanolic extracts of V. negundo leaves.