Utilization of serum proteins by normal and poliovirus infected monkey kidney cells

Abstract

The quantitative utilization of serum protein by epithelial-like cells in monkey kidney cell cultures in vitro has been studied. It was found that all electrophoretically separable serum components were utilized by the cells during the most active growth phase, namely the first 3 days. During the second three days of growth very little protein was utilized. After infection of the cells serum protein utilization was restricted to the first day, namely the period of virus multiplication. A continuous monolayer of epithelial like cells was obtained in 6 days without the 3 day routine fluid change. Since approximately half of the 10 per cent added calf serum was utilized it appears that the addition of 5 per cent calf serum would eliminate the necessity of a fluid change.