Abstract
A specific binding of 3H-actinomycin D (AMD) to DNA localized in plastic-embedded tissues can be obtained by floating directly ultrathin sections on the labeled antibiotic solution. The wash of the sections in organic solvents afterward is necessary in order to obtain a very low unspecific background. The 3H-AMD-binding sites are detected by radioautography. This technique has been applied to various biological materials: rat lymph node, crab testis, normal or virus-infected monkey kidney cells in culture, isolated rat liver nuclei with condensed or dispersed chromatin. The silver grains are always concentrated over the condensed chromatin. Particular localizations observed in some nuclei are discussed. The technique is very simple and could be useful for localizing small amounts of DNA in cells at the ultrastructural level.
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