Abstract
1. A technique using tube cultures of monkey kidney cells for the determination of 50% neutralization titres of immune sera following smallpox vaccination is described. The cytopathogenic centres in the tubes are counted after specific adsorption of chick erythrocytes. 2. It is emphasized that the expression of the vaccinia virus neutralizing activities of immune sera in relation to a standard preparation is necessary for obtaining reproducible titration results in cell cultures of monkey kidney cells. 3. The expression of the vaccinia virus neutralizing activity of immune sera as the proportional relative neutralizing potency is proposed. 4. A pool of sera from adults 6 weeks following primary vaccination is used as a standard preparation. 5. The effect of various factors influencing the 50% neutralization titres observed in cell cultures is discussed. 6. The percentage of relative neutralizing potencies of 22 sera 6 weeks following primary vaccination and of 19 sera 18 years following primary vaccination are determined twofold. 7. Sera 6 weeks following primary vaccination appear to show synergism with respect to vaccinia virus neutralizing action.
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