Abstract
1 * Abstract Biotransformation is the process whereby a substance is changed from one chemical to another (transformed), by a chemical reaction. In the current work, appropriate method was developed to grow fungal pellets in flak batches inoculated with fungal spores, to improve the separation of the biomass from solution and reduce the extraction complications. Also, the capacity of the fungal pellets to be reused for methandienone production using isolated strain of Eupenicillium katenegenes was investigated. The results showed that inoculation with different concentrations of fungal spores could affect the structure of the mycelial pellets, and the methandienone formation. The pellets that were formed after inoculation with fungal spores of 3x103 spore/ml at 150 or 200 rpm showed considerable methandienone formation. This was a matter of permeability of the substrate to reach the cells, mass transfer, and ability of the pellets to withstand the toxic effect of the substrate. Incubation for 48 h guaranteed the formation of amount of methadienone over 95%. Almost, 100% of the biotransformation capacity was retained for four successive cycles. The complete loss of the transformation ability was observed after fourteen cycles. Such promising results using this strain to transform methyltestosterone to the important drug methandienone were recorded here for first time as far as known.
Published Version
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