Abstract
The generation of Fly Ash (FA) waste is continuously piling up with the increasing energy demand. Recent research is focused towards reutilizing this fly ash waste through bioremediation practices. But fly ash retards the growth of plants and holds back to support the bioremediation process due to a deficiency of essential main nutrients. The present research envisages overcoming this problem by providing a novel concept of inducing isolated microbes and treated wastewater which provides necessary nutrients and promotes better plant growth and metal extraction. A pot experimental study was executed with treatments T1 (FA amended soil), T2 (FA with isolated microbe), and T3 (FA with microbes and treated wastewater). As an outcome of the present research, T3 gained relatively higher morphological characteristics viz. Leaf area (29.8%), absolute growth rate (61.7%), plastochron index (18.6%), biomass yield (47.3%) and enhanced metal extraction for Fe (34.4%), Al (27.1%), Mn (72.0%), Zn (17.5%) in comparison to the control. Treatment T3 also gained higher Remediation Efficiency (RE) and Bio-Concentration Factor (BCF) values for Al, Fe, and Mn. The involvement of nutrients via treated wastewater energizes the process mechanism and increases the working zone for the microbes thereby, enhancing the bioremediation.
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