Abstract
Fusarium oxysporum f.sp. Lupini is a common fungal pathogen on lupine plants causing wilt disease resulting in serious economic losses. Therefore, this research was conducted in order to survey the wilt disease in five Governorates. Results showed that isolates obtained from Ismailia were the highest in the pathogenesis ability. Isolation and identification also showed that Fusarium oxysporum f.sp. Lupiniis the main pathogen of this disease. The effect of soaking lupine seeds before planting in different concentrations of antioxidants such as ascorbic acid and citric acid, salicylic acid, sodium benzoate with four concentrations i.e. 25, 50, 100and200ppm, as well as five botanical extracts are leek leaves, thyme, basil, marigold, neem seeds with the concentrations 1, 2,4,6% under greenhouse conditions. Pots experiments results showed that all antioxidants tested, as well as plant extracts significantly reduced the proportion of infected plants with wilt and increased the proportion of the remaining healthy plants, and also the various growth parameters compared to the control treatment. The results showed that the salicylic acid concentration 200ppm gave the best percentage of healthy plants and also the various growth parameters followed by the leek extract. Field results were similar to the results of the greenhouse experience where the each antioxidants tested, as well as plant extracts had increased the healthy plants ratio compared to the control has dovetailed increase in healthy plants percentage with the increase in the weight of the dry seed showing that the treatment of salicylic acid followed by ascorbic acid were the most influential, as well as the treatment of the balls, followed by neem were the best in plant extracts. The antioxidants are the best ever from plant extracts. The results indicate the possibility of using some chemical inducers and plant extracts in wilt resistance in a lupine in Egypt.
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