UTILIZATION OF ALOE VERA GEL AS GROWTH ENHANCER ON MICROPROPAGATION OF EUCALYPTUS CITRIODORA HOOK PLANT

Abstract

This study aimed to examine the influence ofAloe vera gel at different concentrations as growth enhancer on Eucalyptus citriodora, Hook micropropagation. The experiments were conducted from April 1st 2019 until January 15th, 2020 at Plant Tissue Culture Laboratory of Prof. Dr. Abd El-Fatah H. Belal, Fac. Environ. Agric. Sci., Arish Univ. The obtained results presented that, soaking Eucalyptus citriodora explants on (Clorox)® solution at 20% (v/v) for 20 minutes recorded the highest survival percentage of explants (87.33%) on establishment stage. Furthermore, addition of BA at 1 mg l-1 in combination with NAA at 0.1 mg l-1 recorded the highest shoot number/explant, shoot length (cm) and leaf number/shoot (7.33, 4.66 and 9.50, respectively) on multiplication. Also, the maximum shoot number/explant, shoot length (cm) and leaf number/shoot (9.33, 7.00 and 11.50, respectively) were achieved with addition of Aloe vera gel at 20 m l-1. Moreover, Murashige Skoog (MS) medium with IBA addition at 1.0 mg l-1 recorded the maximum growth of Eucalyptus citriodora during rooting stage. i.e., shoot number/explant, shoot length (cm), leaf number/shoot, rooting percentage, root number/explant and root length (cm) (3.44, 8.13, 12.86, 99, 3.27 and 5.07, respectively). Finally, rooted shoots were highly successful (above 80%) on soil mixture contains peatmoss and sand at a rate of (1:1, v:v).