Abstract
A gas-diffusion flow-injection system for the assay of urea is reported. Urea is enzymatically converted to ammonia, which is determined spectrophotometrically by detecting the change in absorbance of a mixed pH indicator. The enzyme is immobilized by addition of perfluoroalkyl chains to the free amine groups of the enzyme and then adsorption of this modified enzyme on a polytetrafluoroethylene gas-diffusion membrane. The method is applicable in the range 0.1–500 mM urea. The procedure was used in comparison assays using blood serum samples.
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