Utilization of acetate-1- 14C for acetylation of an aromatic amine by the guinea pig adrenal gland

Abstract

Guinea pig adrenal slices utilized acetate-1- 14C to acetylate the aromatic amine procaine. Adrenocorticotrophin did not increase the amount of procaine acetate formed. When a high concentration of procaine and a low concentration of acetate1- 14C were added to adrenal slices, no detectable amounts of steroids 14C were formed. The supernatant fraction of the adrenal gland was most active in acetylating procaine, and the addition of the other adrenal fractions decreased the amount of procaine acetate formed by the supernatant fraction. Guinea pig tissues other than the adrenal gland could convert procaine and acetate to procaine acetate.