Abstract
Polyadenylic molecules (poly A) are single stranded nucleic acids containing a series of repeating adenine bases. Polyriboadenylic (poly (rA)) strands form parallel duplexed helices in acidic conditions. Our group has generated and utilized a technique to determine if polyadenylic sequences can pair when each sequence differs in composition. We have adapted a DNA nanoswitch developed by others to detect duplex formation where we can control the composition of each strand that is being tested for interaction. This method is superior to traditional UV melting studies, given the low specificity for detection of duplex species in a complex mixture of polyadenylic strands with different compositions. Our results show that nanoswitches can be used to detect poly(rA) duplex formation as a function of pH and we have identified interesting binding between polyadenylic strands that have different compositions. Our methodology not only provides fundamental information regarding parallel polyadenylic duplex formation, but also can help in design of future and potentially pH‐sensing biological nanotechnology.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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