Abstract
D-Lactose can be used by a majority of bacteria in culture but can not be utilized by most plant cells and tissues in vitro. D-Lactose has been substituted for sucrose in Linsmaier and Skoog agar medium and was found to promote the growth of tobacco tumor tissue induced by Agrobacterium tumefaciens strain B6S3. The growth of this tumor tissue was stimulated by a factor of 2.3 after 12 days of culture. However, tumor calli induced on tobacco by strain C58 of Agrobacterium tumefaciens as well as the normal wild type calli (NW) can not grow at all on the same medium. In contrast to D-lactose, D-galactose did not stimulate growth of either of the tumor tissues. Possible explanations for the growth of B6S3 tumor calli on agar medium supplemented with D-lactose are discussed.
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