Abstract
Isotachophoretic separation of free amino acids using Cu 2+-diethylene triamine (Den) in high-pH leading electrolytes is investigated. Large changes in relative step heights of free amino acids are seen. Mobility order depends on both Cu 2+ concentration and Cu 2+/Den ratios. Mobility differences seen with CuDen addition can be ascribed to both polyvalent counterion effects and specific interactions forming tertiary complexes. These electrolytes offer a new dimension for mobility variation of complexing amino acids and peptides.
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