Abstract
Aim:The present study was undertaken to detect the presence of canine parvovirus (CPV) in fecal samples of diarrheic dogs by conventional polymerase chain reaction (PCR) and immunochromatographic (IC) strip test and to compare the diagnostic potential of these tests.Materials and Methods:A total of 50 fecal samples collected from diarrheic dogs suspected for CPV infection were subjected to PCR using CPV-555 primer amplifying the gene coding for the VP1 protein. These samples were also tested by IC strip test using a commercial rapid Ag test kit. The results were statistically analyzed using McNemar test.Results:A total of 22 samples (44%) were detected as positive by PCR, which yielded a specific amplicon of 583 bp. In IC strip test, 18 (36%) samples were found to be positive. The sensitivity of the test as compared to PCR was found to be 72.22% and specificity was 92.86%. Positive predictive value and negative predictive value of IC strip test was found to be 88.89% and 81.25%, respectively. Statistical analysis of the results of PCR and IC assay using McNemar test revealed no significant difference (p>0.05).Conclusion:The IC strip test could be employed as a rapid field level diagnostic tool for the diagnosis of canine parvoviral diarrhea.
Highlights
Parvoviral enteritis is a highly contagious disease causing heavy mortality in dogs all over the world
Materials and Methods: A total of 50 fecal samples collected from diarrheic dogs suspected for canine parvovirus (CPV) infection were subjected to polymerase chain reaction (PCR) using CPV-555 primer amplifying the gene coding for the VP1 protein
The IC strip test could be employed as a rapid field level diagnostic tool for the diagnosis of canine parvoviral diarrhea
Summary
Parvoviral enteritis is a highly contagious disease causing heavy mortality in dogs all over the world. And rapid diagnosis is quintessential, so that the infected dogs can be isolated and supportive treatment can be adopted to reduce morbidity and mortality [1]. The infection is characterized by acute hemorrhagic gastroenteritis in adult dogs and myocarditis in puppies causing high morbidity and mortality [3]. Diagnosis on the basis of clinical signs is not definitive, since several other pathogenic organisms can cause diarrhea in dogs. The standard method for the identification of CPV infection is the detection of morphologically intact virus particles by electron microscopy (EM). This method is fast and specific, but expensive and less sensitive [4].
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