Abstract
When isolated rat fat cells are incubated with radioactively labelled palmitate the extracellular fatty acid pool is diluted by fatty acids of endogenous origin. Using fat cell concentration as an experimental variable, a simple graphical procedure has been devised to correct for the difficulty such precursor dilution causes in measurement of oxidation and esterification of exogenous fatty acids. After application of such corrections it was calculated that extracellular palmitate was utilised for oxidation with a composite K m of approx. 7 μM. The composite K m for palmitate esterification was 29 μM (no other substrates present, albumin included at 10 mg/ml).
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