Abstract
Oocyte quality is pivotal for fertilization and early embryonic development. Ustiloxin A (UA), is an emerging mycotoxin that has been frequently detected in rice and paddy. Because UA has been reported to be phytotoxic and cytotoxic, it poses a potential hazard to human and animal health. However, the effects of UA on oocyte maturation remain unknown. Here, we investigated the effects of acute UA exposure on mouse oocyte maturation. First, UA exposure inhibited oocyte maturation in a concentration-dependent manner and induced meiotic arrest by disrupting spindle assembly and reducing actin density. Moreover, mitochondrial function was substantially disrupted in oocytes upon UA exposure. Aberrant mitochondrial distribution, substantial downregulation of mitochondrial dynamics-associated genes Mfn1, Mfn2 and Fis1, decreased membrane potential and TOM20 expression were observed in UA-exposed oocytes; these effects further led to oxidative stress and DNA damage. Furthermore, UA induced ER and Golgi dysfunction and triggered ER stress by increasing GRP78 expression, which ultimately resulted in autophagy and early apoptosis in oocytes. Therefore, these results demonstrate that UA impairs oocyte quality by disrupting organelles function, providing new insight into the influence of UA on female reproduction in mammals.
Published Version
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