Abstract
This study was carried out for direct detection of typhi and some of its multidrug resistance genes(tem,capt,gyrA&sul2)which encode for resistance to (Ampicillin, Chloramphenicol,Ciprofioxacin,Co-trimoxazole)by using Polymerase Chain Reaction technique .(71)blood samples for people suffering from typhoid fever symptoms depending on the clinical examination and (25)for control were collected. The results investigation for flic gene which encode for flagellin protein indicated that only (19)with percentage of (26,76%)gave appositive results while all control had a negative ones. Investigation for antibiotic resistance drug in samples which show positive results for flic gene showed that there is a multidrug for all antibiotics with (94.73%) in patients
Highlights
الكشااف عاان الجين ااا )9tem,capt,gyrA,sul المماوداااااة ل م اااااا ا الحيوياااااة ، Ampicillin Co-، ،Ciprofloxacin، Chloramphenicol ) ،trimoxazoleو ع ى التاوالل فال هاذه البكترياا
This study was carried out for direct detection of typhi and some of its multidrug resistance genes(tem,capt,gyrA&sul2)which encode for resistance to (Ampicillin, Chloramphenicol,Ciprofioxacin,Co-trimoxazole)by using Polymerase Chain Reaction technique .(71)blood samples for people suffering from typhoid fever symptoms depending on the clinical examination and (25)for control were collected
Investigation for antibiotic resistance drug in samples which show positive results for flic gene showed that there is a multidrug for all antibiotics with (94.73%) in patients
Summary
الكشااف عاان الجين ااا )9tem,capt,gyrA,sul المماوداااااة ل م اااااا ا الحيوياااااة ، Ampicillin Co-، ،Ciprofloxacin، Chloramphenicol ) ،trimoxazoleو ع ى التاوالل فال هاذه البكترياا والأه ي ااة يعتم ااد ع اااى ال ااتلازن ب ااين المست ااادا والأ اادا وهااذا لا يح(اا ملا بعااد ظهااور أ اادا المست ااد )0خاالال )8-6أيااام وظهااور أ اادا المست ااد ] [Hخاالال )22-29يااوم )15وهااذا بالتاالل ياو ي ملاى تافخر الكشاف عان الإصاابة ياا اساتعجال المارض لاذا فاان ت(افية PCRلتط ا سااعا ق ي اة فماب بعاد اساتولاص ألادنا ل ح(اول ع ى دع ودا ) (16تا استودام العينا )22التال أعطت نتيجة دوجبة فل اختبار flic geneل تحاري عن الجينا المماودة ل م ا ا الحيوية فال بكترياا
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