Using Surface Plasmon Resonance Spectroscopy To Measure the Association of Detergents with Self-Assembled Monolayers of Hexadecanethiolate on Gold

Abstract

This paper describes the use of surface plasmon resonance (SPR) spectroscopy to measure the rates and extents of association of four detergentssodium dodecyl sulfate (SDS), β-octyl glucoside, Triton X-100, and Tween 20to self-assembled monolayers (SAMs) of alkanethiolates on gold. SAMs presenting hexaethylene glycol groups resisted the adsorption of all four detergents. These same detergents associated with hydrophobic SAMs presenting methyl groups; the concentration of detergent molecules on the surface was 120−280 pmol/cm2. The associations of the detergents with the hydrophobic SAM were described well by the Langmuir adsorption isotherm. The dissociation constants Kd (M) for the desorption of the detergents from the surface correlated with the critical micelle concentration (cmc, M) of the detergents in solution, and followed the relationship cmc ≈ 7 (±2)Kd. The efficacy of SDS in removing the protein fibrinogen adsorbed on a hydrophobic SAM depended strongly on the concentration of detergent. SDS at a...