Abstract

This paper demonstrates that surface plasmon resonance (SPR) spectroscopy can be used to measure the nonspecific adsorption of proteins to self-assembled monolayers (SAMs) of alkanethiolates on gold in situ and in real time. Mixed SAMs comprising hexa(ethylene glycol) and methyl groups that have values of XMe 0.5 : the amount of adsorbed protein correlated with XMe. The initial rate for adsorption of fibrinogen to a methyl-terminated SAM (XMe = 1.0) followed first-order kinetics. The combination of SAMs and SPR described here is particularly well suited for investigations of the interactions of proteins with structurally well-defined organic surfaces.

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