Abstract

The aim of this study was to develop polymerase chain reaction (PCR) assay for specificdetection of low levels of bovine in meat products. Oligonucleotide primers were designed foramplification of species-specific mitochondrial DNA sequences. When applying the assay to DNAextracts from different meat products samples that were prepared from binary meat mixtures, it waspossible to detect each species or it was possible not to detect the meat species that the label indicate. Inconclusion, it can be suggested that this PCR can be used to determine mislabelled and/or fraudulentspecies substitution in comminuted meat products.

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