Using δFIP as a potential biomarker for risk assessment of environmental pollutants in aquatic ecosystem: A case study of marine cyanobacterium Synechococcus sp. PCC7002

Abstract

Increased hazardous substances application causes more environmental pollution and risks for human health. Microalgae are the important biological groups in marine ecosystem, and considered to be sensitive to environmental pollutants. Therefore, toxicity test on marine microalgae could provide the most efficient method for aquatic toxicity assessment, and could also be used as the early warning signals in aquatic ecosystem. In view of this, our study aimed at investigating the toxicity potential of two typical organic compounds, and screening out novel photosynthetic indicators for the risk assessment of environmental pollutants. In this study, benzyl alcohol and 2-phenylethanol were chosen as the target organic compounds, and preliminary toxicity mechanism of these organic compounds on marine cyanobacterium Synechococcus sp. PCC7002 was investigated with chlorophyll fluorescence technology. Results showed that PCC7002 could be affected by benzyl alcohol or 2-phenylethanol stress, and the toxicity effect was concentration-dependent. And external benzyl alcohol and 2-phenylethanol stress damaged the oxygen evolving complex, and suppressed electron transport at the donor and receptor sides of photosystem II (PSII), influencing the absorption, transfer, and application of light energy. Furthermore, potential biomarkers were screened by half maximal inhibitory concentration (IC50) on the basis of pearson correlation coefficient analysis, and fluorescence intensity difference between the I-step and P-step of OJIP curve (δFIP) seems to be the most sensitive indicator for external stress. This study would be of significant interest to the biomarker community, and pave the way for the practical resource for marine pollution monitoring and assessment.