Abstract
Premise of research. The population genetics approach is an important tool for determining boundaries in closely related plant species. Here, we adopt this approach to investigate the relationship between three species of Herbertia endemic to the Pampa biome, a complex grassland environment in southern South America.Methodology. A multianalytic approach based on population genetics was used to analyze 102 intersimple sequence repeat loci sampled in 11 populations (333 individuals) unequivocally attributed to Herbertia, aiming to test the boundaries between species. Controlled pollination experiments were performed to estimate the index of self-incompatibility (ISI), and the viability of pollen grains was estimated using the Alexander dye method, aiming to determine the breeding system in studied populations.Pivotal results. Bayesian analysis revealed three unambiguous genetic pools that corresponded to the species Herbertia darwinii (94 individuals), Herbertia pulchella (111 individuals), and Herbertia qu...
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