Abstract
Albendazole (ALB) is a potent benzimidazole anthelmintic used in the treatment of human intestinal helmintiasis as well as of hytatid cysts and neurocysticercosis. Two rapid, simple, sensitive, and selective spectrophotometric methods are presented for the determination of ALB in pharmaceuticals. The methods are based on the formation of dichloromethane soluble 1 : 1 ion-pair complexes (ALB : dye) formed between ALB and sulfonphthalein dyes, bromophenol blue BPB, (method A) and bromothymol blue BTB, (method B). The complexes formed were measured directly (without extraction) at 445 nm (method A) and 460 nm (method B). The experimental conditions were optimized and the systems obey Beer’s law for 1.5–21.0 and 2.0–32.0 μg mL−1 ALB for method A and method B respectively. The molar absorptivity and Sandell's sensitivity were calculated to be L mol−1 cm−1 and 0.0209 ng cm−2, and L mol−1 cm−1 and 0.0350 ng cm−2 using BPB and BTB, respectively. The limits of detection and quantification were calculated to be 0.01 and 0.03, and 0.16, and 0.49 μg mL−1 using BPB and BTB, respectively. The relative standard values for intra-day and inter-day precision were less than 3%, and the accuracy was better than 3% for both methods.
Highlights
Albendazole (ALB), chemically known as methyl-5-(propyl thio)-2-yl benzimidazole methyl carbamate [1], is widely used as an anthelmintic having a wide spectrum of activity [2]
Two more indirect methods [31] involving NBS-metol-sulfanilamide and NBScelesteine blue as chromogenic systems have been proposed by Sastry et al Several other reaction schemes involving chloramine-T-methyl orange or indigocarmine [6], chloramineT-metol-sulfanilic acid [7], NBS-methyl orange or indigocarmine [10], N-chlorosuccinimide-iron(II)-thiocyanate [11], NaIO4-KBr-methyl orange or indigocarmine [12], and perchloric acid-crystal violet [4] are found in the literature for the indirect spectrophotometric assay of ALB in its dosage forms
The results showed that there is no difference between the proposed methods and the official method with respect to accuracy and precision
Summary
Albendazole (ALB), chemically known as methyl-5-(propyl thio)-2-yl benzimidazole methyl carbamate [1], is widely used as an anthelmintic having a wide spectrum of activity [2]. Sastry et al [37] have devised extractive spectrophotometric methods for the estimation of some benzimidazole anthelmintics including ALB in pharmaceuticals based on ion-pair reaction and employing some acidic dyes. In an attempt to overcome these shortcomings associated with extractive spectrophotometric methods, several workers in recent years have devised extraction-free spectrophotometric methods for several pharmaceutical substances [38,39,40,41,42,43,44,45] based on ion-pair reaction and employing some sulfonphthalein dyes These new procedures are free from critical pH adjustment and laborious liquid-liquid extraction step and involve simple mixing the drug and dye solutions in organic solvent and measuring the absorbance of the resulting ion-pair complex at wavelength of maximum absorption. They were demonstrated to be highly precise and accurate besides being robust and rugged
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