Use of the Sulfur-Containing Growth Regulator Tiaton for Rooting Micro-Gears of Grapes in Culture in vitro

Abstract

The study was carried out to evaluate the effect of a nutrient solution for the rhizogenesis of grape micro–gears in the Murasiga-Skuga medium (MS) with the addition of various concentrations of the drug Tiaton (0.1, 0.15, 0.20 and 0.25 ml/l). In experiments, the effect of different concentrations of the drug Tiaton on the rooting and vegetative growth of grapes of the Khasansky and Moskovsky White hybrids in vitro was studied. When the Tiaton preparation was added to the MS nutrient medium in various concentrations, the rooting capacity of the micro-gears of the Khasansky hybrid grape increased by 40%, the total root length increased by 2.4 times compared with the control. In the Moscow White hybrid, when using the Triaton growth regulator, an increase in the rooting capacity of micro gears up to 25% and the total root length by 1.5 times is shown. The optimal concentrations of the Triaton preparation for addition to the MS nutrient solution for optimal rhizogenesis of micro-gears and the development of vegetative growth of cuttings of the studied grape hybrids have been established. Thus, for Khasansky hybrid grapes, the most optimal medium for improving rooting and root development of micro gears was ½ MS + 0.15 ml/l of Tiaton preparation. In the Moscow White hybrid, a significant increase in the rooting capacity of micro-gears and good development of the root system was obtained when Tiaton was added to the nutrient medium for rhizogenase of micro-gears in concentrations (0.15 and 0.20 ml/l).