Abstract
The aim of this study was to detect the efficacy of some plant extracts in protection of liver cells from toxic effects of anti-cancer drug Methotrexate by using metabolizing enzymes glutamic oxaloacetic transaminase (GOT), glutamic pyruric transinase (GPT), and alkaline phosphatase (ALP), as parameters. Aquatic and alcohclic extracts were prepared from the Seeds of Nigella sativa, (Citrus aurntifolia) and cardamom (Elptteria cardamam ), for each extract, four gradually increased doses were evaluated for their toxicity. Dose with no effect was selected for further investigation. Drug-plant extract interaction was conducted using white mice musculus Balb/c strain. The animals were divided into three groups: negative controls (buffer), positive control (drug) and interaction group (drug & plant extract). The results showed that the dose 200 mg/kg, bw of N. sativa seeds extract and citrus fruit extract, as well as 50 mg/kg of cardamom extract induced non significant reduction in activity of GOT, GPT and ALP. However, MTX at dose of 3.25 mg/kg caused a significant (P< 0.01) reduction in those enzymes. Interaction between the selected doses of drug and plant extract, before and after drug treatments, was conducted. Significant reduction of drug effects on GPT, GOT and ALP was noticed when N. sativa and cardamom extracts were given for 7 days before drug treatments. Citrus fruit and aquatic extract of cardamom were negative in such experiment. Patient on treatment with MTX should be checked for liver function test and these plants might protect him.
Highlights
Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line
Streptomycetes have considerable medical, biological and commercial importance [1], since they produce over 60% of known antibiotics and many other substances with valuable clinical and other applications [2]. Various antitumor compounds are produced by streptomycetes, which have activities against different types of tumor cells [3,4,5]
Ninety-six well tissue culture plates were used for this test to ensure the toxic effect of crude extract of fermentation culture broth of SRY-3, SRY-25, 5b, 10, NS38 on plasmacytoma SU 99 cell line
Summary
Microorganisms: Local isolates of Streptomyces SRY-3, SRY-25, 5b, 10, NS-38 were used throughout this work. Ninety-six well tissue culture plates were used for this test to ensure the toxic effect of crude extract of fermentation culture broth of SRY-3, SRY-25, 5b, 10, NS38 on plasmacytoma SU 99 cell line. The wells of the late four lines in the same plate were filled with 0.2ml tissue culture medium only considered as a control. A 0.15ml cell suspension of plasmacytoma cell culture (104 cells per ml) was added to Analysis of bleomycin by HPLC: The quantitative and qualitative assays of bleomycin were performed by high performance liquid chromatography (HPLC) the eluents were injected into the HPLC: column: shimi – pock Antimicrobial activity: Block assay method was used to determine the antimicrobial activity of Streptomyces isolates grown on scarped and eluted with methanol and dried for further investigation. Antibiotic Susceptibility: Antibiotic resistances of Streptomyces isolates were examined by using antibiotic sensitivity discs [19]
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