Abstract
The glutamic oxalacetic transaminase (GOT) and lactic dehydrogenase (LDH) activities of spermatozoa and seminal plasma were determined for control (25 °C), cold-shocked (0 °C), and deep frozen (−196 °C) boar semen. Semen from four ejaculates of each of four boars was used. Freezing caused significant ( P < 0.01) losses of GOT and LDH activities from the spermatozoa and concomitant significant ( P < 0.01) increases in the seminal plasma. The magnitude of enzyme losses from spermatozoa was greater for GOT (68.7 to 86.2%) than for LDH (22.6 to 31.8%). In addition to “leaching” of enzymes from sperm cells into seminal plasma, some inactivation of LDH occurred during freezing. Cold shock caused significant ( P < 0.05) losses of GOT and LDH activities from the spermatozoa. Dilution of boar semen with an egg yolk extender slightly reduced the loss of GOT and LDH activities occurring during cold shock. The data indicate that GOT activity may be a more useful parameter than LDH activity for assessing boar sperm cell damage during freezing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
