Use of Simple Sequence Repeat Marker to Assess the Segregating Population of Rice (Oryza sativa) in field for Grain Length Quality Parameter

Abstract

Rice grain quality is of paramount importance in the consumer preference for rice varieties and therefore the development of rice varieties should match to the consumer preference. Assessment of segregating population of breeding material in rice for quality parameter made it possible by the use of simple sequence repeat(SSR) marker. The F2 mapping population of a cross between a long slender rice genotype VDN 1137-1-1 and a short slender rice landrace Badshabhog was studied for the inheritance of grain length and to identify the marker associated with grain type. The F2 population showed 3:1 ration for long slender to short slender grain type. This segregating population was assessed by 24 SSR markers using bulk segregant analysis. Among these 24 SSR Markers, a marker RM 147 reported polymorphism between the long slender and short slender parents and respective bulks. Genetic analysis indicated goodness of fit for single gene model indicating the association of RM147 with grain length gene in the segregating population. Therefore, SSR marker RM 147 is of immense value in the assessment of grain quality parameter in rice during the development of rice varieties.