Abstract
Inbred lines are important germplasm in cauliflower breeding programs. To understand the genetic diversity and relationships of cauliflower inbred lines, the use of simple sequence repeat (SSR) markers will be of great value for parental line selection and breeding strategy design. In this study, the genetic diversity and relationships of 165 cauliflower inbred lines primarily derived from southeast China were assessed using SSR markers. Forty-three SSR markers were polymorphic across these inbred lines and generated a total of 111 alleles. The mean values of the number of alleles (Na), effective number of alleles (Ne), Shannon’s Information index (I), and polymorphism information content (PIC) per locus were 2.581, 1.599, 0.517 and 0.316, respectively. Genetic distance values among all pairs of the inbred lines varied from 0 to 0.67 with an average of 0.30. On the basis of genetic distance data estimated with the SSR markers, the 165 cauliflower inbred lines were classified into four main clusters (from group Ⅰ to group Ⅳ) by cluster analysis and four subpopulations (from POP 1 to POP 4) by structure analysis. The classification patterns of most cauliflower inbred lines were not consistent with their curd maturity, curd solidity or geographic origins. These results based on estimates by the SSR markers, suggested the genetic diversity of the 165 cauliflower inbred lines was relatively narrow. Therefore, pyramiding the valuable genes among different types of the cauliflower inbred lines is important to increase the genetic diversity to obtain desirable hybridization combinations. The information generated in this report will be useful for assessing germplasm and breeding in cauliflower.
Highlights
Cauliflower (Brassica oleracea var. botrytis) is one of the important horticultural crops with an annual global production of over 25 million tons [1]
The genetic diversity and relationships of cauliflower inbred lines assessed by using simple sequence repeat (SSR) markers agronomic characters, and some elite inbred lines have been used in cauliflower breeding programs
The parameter values of number of alleles (Na), effective number of alleles (Ne), Shannon’s Information index (I), and polymorphism information content (PIC) per locus were used to estimate genetic diversity, which are listed in S2 Table
Summary
Cauliflower (Brassica oleracea var. botrytis) is one of the important horticultural crops with an annual global production of over 25 million tons [1]. Botrytis) is one of the important horticultural crops with an annual global production of over 25 million tons [1]. As an economical and nutritional vegetable crop, cauliflower is widely cultivated in India, China, Ecuador, America, Spain, and Mexico, among others. Cauliflower has received much attention by breeders to improve. The genetic diversity and relationships of cauliflower inbred lines assessed by using SSR markers agronomic characters, and some elite inbred lines have been used in cauliflower breeding programs. Inbred lines are important breeding materials in cauliflower breeding programs. An understanding of the genetic diversity and relationships of cauliflower inbred lines will be useful for assessing germplasm and breeding in cauliflower [2,3]
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