Use of molecular markers in detection of synonymies and homonymies in grapevines ( Vitisvinifera L.)

Abstract

RAPD and microsatellites are used in the present study as molecular markers for characterisation of grapevine germplasm material. The studied varieties were located in two germplasm banks in Spain. Thirty nine accessions were included in the study, including some presumed synonymies and homonymies. Sixty six primers were used for the RAPD study. Twenty were selected for the multivariate analysis and grouping of the varieties. Seven varieties had a monotypic pattern. Twenty three accessions were also analysed with microsatellites with the VVMD7, VVS2, VVS5 and VVS29 loci, obtaining eight different patterns. The high discriminating ability of the RAPD analysis allowed all the studied varieties to be distinguished. A good concordance was obtained for both RAPD and microsatellites when the two analysis were carried out. The following synonymies were confirmed: Moristell and one of the accessions of Monastel; Maturana and Ribadavia; Concejón and one of the accessions of Monastrell; and most of the studied muscat varieties. Homonymies were detected for Miguel de Arco, Monastel, Monastrell and Turruntés. Both RAPD and microsatellites are considered as adequate molecular markers for characterisation of germplasm banks with the use of one or the other depending on the objectives of the study. When results are intended to compare with other laboratories or germplasm banks, microsatellites give simple and more comparable results.