Abstract
Lipoxygenase derived metabolites of linoleic acid generated by incubation with protein extracts from maize embryos treated with abscisic acid have been analysed by thermospray high-performance liquid chromatography-mass spectrometry (HPLC-TSP-MS). TSP-MS data for various isomeric α-and γ-ketols, ketodiols and trihydroxyacids are reported. The molecular weight and the minimum number of oxygenated functions in the structures can be readily characterized from the TSP spectra obtained in the positive and negative ion modes of acquisition. TSP analysis of the methoximated compounds allows the characterization of ketone or aldehyde groups. Additionally, the methoximated derivatives show abundant ions derived from concomitant losses of methanol and the breakdown of the CC bond α to the methoxime. These ions are the base peaks in the TSP spectra of compounds bearing an α-ketol moiety in their structures. The TSP data and fragment assigments are in agreement with the structures previously elucidated by gas chromatography (GC)-MS techniques. Furthermore, the characteristic fragmentation pattern of the methoxime derivatives allows the characterization of a pair of positional isomer α-ketols not detected previously by GC-MS. Some of these metabolites have not been described before in maize
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.