Abstract
We assayed methyl and ethyl acetate as organic modifiers for mobile phases used in reversed-phase high-performance liquid chromatography for the removal of impurities from steroids of pharmaceutical interest. By using ternary mobile phases consisting of the esters, water and a third component that was miscible with both and applying systematic experimental methodology, we developed an optimal procedure for the isolation of the compound of interest (9α-fluoroprednisolone acetate) from the impurities resulting from its synthesis in a minimum time. Inclusion of an acetic ester in the reversed-phase high-performance liquid chromatographic mobile phase was found to improve the resolution of chemically similar subtances.
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