Use of metallic nanoparticles for characterization of muscle tissue by electrical impedance spectroscopy (EIS)

Abstract

Objectives. The electrical impedance spectroscopy (EIS) is relatively new technique used in medicine. The main problems that should be solved are its low resolution and that it fails to distinguish between tissue types, so some kind of the contrast should be applied. Magnetical nanoparticles have been used for imaging and other medical applications. For that reason, our research group decided to analyse the changes of electrical properties of chicken muscle tissue caused by three different types of metal nanoparticles at 50KHz. Methodology. Bio-Logic Science Instruments SP-150 was used as EIS device. Three different particles were analysed: two types of nanomagnetite (NM1 and NM2) and one of Gold particles (GNP). NM1 and NM2 samples were synthetized by coprecipitation and combustion method, respectively. GNP were synthetized by Turkevich method. Nanoparticles were characterized by SEM and RAMAN spectroscopy. Four needles were placed in each chicken breast to connect the EIS device. Measurements were obtained from each chicken breast at basal stage and after being injected with nanoparticles. Data was analyzed by bode graphics (module and phase). Contribution. The major changes of electrical properties of tissue were evidenced by using NM1 and GNP.