Abstract

Abstract Analysis of a sample of clinical or biological origin often requires assay procedures that are rapid, sensitive, selective, and permit the use of microsamples. These constraints have prompted the clinical chemist and analytical biochemist to use many modern tools of the analytical chemist—including atomic absorption spectroscopy, gas chromatography, and luminescence spectroscopy. This review considers the impact of molecular luminescence spectroscopy (fluorescence and phosphorescence) in the analysis of samples of clinical and biological interest. We attempt to outline those procedures and applications of luminescence spectroscopy which are well-established and also consider the promising new developments which should permit wide levels of acceptance in the near future.

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