Abstract
It is well established that selective agar and broth media are necessary for recovering Clostridium difficile from stool specimens or rectal swab specimens [1-4]. These methods were used for detecting C. difficile in rectal swab and stool specimens collected from surgical patients at the Beth Israel Hospital in Boston as part of an epidemiologic study of C. difficile. During the course of this study, we discovered that occasionally culture of a swab specimen did not yield C. difficile whereas tissue culture assay of a stool specimen taken from the same patient on the same day was positive for cytotoxin B. We also noted cases in which culture of a swab
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