Abstract
Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on celery have been conducted so far. In this study we made use of simple sequence repeat (SSR) markers generated from previous celery transcriptome sequencing and attempted to detect the genetic diversity and relationships of commonly used celery accessions and explore the efficiency of the primers used for cultivars identification. Analysis of molecular variance (AMOVA) of Apium graveolens L. var. dulce showed that approximately 43% of genetic diversity was within accessions, 45% among accessions, and 22% among horticultural types. The neighbor-joining tree generated by unweighted pair group method with arithmetic mean (UPGMA), and population structure analysis, as well as principal components analysis (PCA), separated the cultivars into clusters corresponding to the geographical areas where they originated. Genetic distance analysis suggested that genetic variation within Apium graveolens was quite limited. Genotypic diversity showed any combinations of 55 genic SSRs were able to distinguish the genotypes of all 30 accessions.
Highlights
Celery (Apium graveolens L.) is a biennial species from the family of Apiaceae with 2n = 2x = 22 chromosomes
We developed a set of EST-simple sequence repeat (SSR) markers [27] through transcriptome sequencing
The objectives of the present work were to: (1) test marker polymorphism on a set of celery cultivars; (2) assess the genetic variation existing in the materials used; (3) detect the genetic diversity and population structure of these materials and (4) explore the efficiency of the primers used for cultivar identification
Summary
Genotyping with molecular markers is used for identification of cultivars [10,11], cultivar fingerprinting [12,13], detection of genetic variation and genetic diversity [14,15,16], construction of linkage maps [17,18,19], mapping genes of interest, and for marker assisted selection (MAS) [20,21,22,23] These researches are frequently carried out with SSR markers for their co-dominant and multi-allelic nature, which makes them more informative than dominant-types of markers. (2) assess the genetic variation existing in the materials used; (3) detect the genetic diversity and population structure of these materials and (4) explore the efficiency of the primers used for cultivar identification
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