Abstract
Several approaches for detection and diagnosis of plant viruses can be used. Despite to their usefulness, some of them have been time consuming and particularly serological tests have been depend upon providing antisera. In recent years obtaining easy, fast and accurate results in detection and determination of viruses has been possible by the development and improvements of molecular techniques. One of the most important methods is the isolation and analysis of replicative double- stranded RNA (dsRNA) from virus-infected plants. The presence number, size, and abundance dsRNAs in infected plant tissue are of potential value for diagnosis. Cellulose-column chramatography are used for purification of viral dsRNA and the presence of dsRNA are represented by agarose gel electrophoresis.
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