Abstract
In this study antibodies against the fragment 254-274 from gp120 of human immunodeficiency virus (HIV) were produced in cats, rats and chicken that can be used as reagents in the preparation of diagnostic kits. Enzyme linked immunosorbent assay showed the presence of anti-HIVgp120 antibodies. Dot blot analysis was used as a separation technique and confirmed the results, proving its efficiency in the detection of proteins. This study also demonstrated that orally given antibodies to an animal as oral vaccine, initiated immune responses of the antibodies in the animals which may be useful for protection, antibody purification and development of reagents for immunodiagnostic procedures.
Highlights
A dot blot is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins
Production of anti-human immunodeficiency virus (HIV) antibodies in animals is a safe alternative in the development of reagents to be used in enzyme-linked immunosorbent assays (ELISA), western blotting and dot blots used to diagnose HIV infections in human [2]
The results of the study is depicted in table 1 that shows the ELISA giving a positive reaction for anti-HIV antibodies in samples from chickens, cats and rats as they all developed color reactions
Summary
A dot blot is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins. It represents a simplification of the Northern blot, Southern blot, or Western blot methods. The technique offers significant savings in time, as chromatography or gel electrophoresis, and the complex blotting procedures for the gel are not required. It offers no information on the size of the target biomolecule [1]. It is an alternative to the use of human antibodies purified from the serum of HIV positive patients for the development of diagnostic kits. The purified antibody could be used in the diagnosis or as a new avenue of therapy for HIV+ patients
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