Abstract
Summary The ploidy level of 10 diploid and 10 induced triploid tench, Tinca tinca, was verified using flow cytometry to determine relative DNA content of DAPI-stained erythrocyte nuclei. The C-value (haploid nuclear DNA content; pgDNA nucleus−1) of these same individuals was determined by means of Feulgen image analysis densitometry, in comparison to the chicken (Gallus gallus domesticus; 1.25 pg P < 0.05) standard, using three different approaches. Highly similar mean C-values were obtained, thus confirming the possibility of using tench blood as a frequently used standard in European pond aquaculture for ploidy determination in fishes.
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