Use of antigen prepared from in vitro culture ofPlasmodium falciparum for the detection of antibodies in sera from patients exposed toPlasmodium vivax

Abstract

The blood stage parasites frominvitro cultures ofPlasmodiumfalciparum were employed for the detection of malaria antibodies in patient sera havingP.vivax infections employing indirect fluorescent antibody (IFA) test. The antibody titres obtained for theP.vivax infected sera ranged from 1∶128 to 1∶1024. Control slides smeared with non-immune sera did not show any fluorescence. On the basis of the results of the present study it maybe concluded that antigen prepared frominvitro cultures ofP.falciparum cross-reacts withP.vivax antibodies.