Abstract
Abstract This paper describes a new approach to maximize sample throughput in an analytical column for the isolation of dilute peptidic compounds. This approach exploits the chromatographic behavior of peptidic compounds which is a large change in retention relative to the change in mobile phase strength. A a result, a small decrease in solvent strength gives added retention without the loss of solubility of the sample. This same behavior also allows the use of an injection solvent which is only slightly weaker than the mobile phase to concentrate the peaks at the head of the column in a solvent overload mode. A short column allows the use of large capacity factors while maintaining reasonable elution times. In addition, cleanup of the column can be accomplished by a single injection of strong solvent in the large loop. This approach was applied to the isolation and identification of degradation products of a peptidic elastase inhibitor, ICI 200 880.
Published Version
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