Abstract
Our new technique allows the decline in ambient ammonium concentration to be followed continuously during phytoplankton uptake experiments. A self-cleaning, in-line filter permits the continuous separation of cells from the medium for nutrient determination by an autoanalyzer. The technique works well with laboratory cultures of the marine diatoms Thalassiosira pseudonana and Skeletonema costatum. Changes in uptake rate on time scales of 1 min or longer can be resolved, the resolution being limited by the response characteristics of the autoanalyzer.
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