Use of a nano-porous gold film electrode modified with chitosan / polypyrrole for electrochemical determination of metronidazole in the Presence of Acetaminophen

Abstract

This study, it was aimed to provide a sensitive, easy and selective method for designing Nano-porous gold film electrode (NPGF) electrode for simultaneous measurement of metronidazole (MT) and acetaminophen (AC). For this purpose, the NPGF electrode surface was modified with chitosan (CS) and poly pyrrole (PPY) by electrochemical method, and then CS and PPY modified NPGF (PPY-CS-NPGF) electrode were used to measure these drugs. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) are employed for the characterization of the attained PPY-CS-NPGF electrode. Using cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and chronoamperometry, the electrochemical behavior of MT was investigated with the modified electrode. By differential pulse voltammetry, linear ranges of concentration 0.005–100 μM with linear coefficients of 0.9898 and a detection limit of 0.0009 μM were obtained for MT. Finally, an electrochemical sensor was used to measure MT in a real sample, which yielded acceptable results. PPY-CS-NPGF electrodes have a wide linear range, high selectivity, sensitivity and stability and can be used successfully to determine these drugs.