Assessing the removal of heavy metals and polycyclic aromatic hydrocarbons and occurrence of metal resistance genes and antibiotic resistance genes in a stormwater bioretention system

Abstract

Bioretention basins are extensively used in urban areas to manage stormwater by reducing peak flows and pollution. This study evaluated the performance of a bioretention basin in removing heavy metals, polycyclic aromatic hydrocarbons (PAHs), and oil and grease. Using droplet digital PCR (ddPCR), the presence of metal resistance genes (MRGs) and antibiotic resistance genes (ARGs) in the basin's soil was analyzed. The results indicated effective removal of Zn (67%), but higher concentration of Mg was observed at the outlet. Cu, Fe and Pb showed no significant differences in the in- and outflow concentrations. The system successfully removed 82% of influent PAHs. Soil samples collected in summer and fall revealed higher MRG abundance in summer, with copA being the most prevalent MRG (1.2–4.8 log10 copies/g soil). Among the ARGs, sul1 was consistently found throughout the basin (2.5–6.7 log10 copies/g soil), while tetW was detected primarily at the basin's start and end in the topsoil layer. Rubellimicrobium and Geobacter were identified as potential carriers of ARGs/MRGs. Although the concentration of metals in soil was not measured in the current study, these findings emphasize the need to understand heavy metal distribution and the occurrence of MRGs and ARGs in stormwater control systems to improve their design and effectiveness.