Abstract
Membrane trafficking is essential for multiple aspects of plant growth and development. Some small molecules have been widely used to study the mechanisms of membrane trafficking in plants. For example, short-term treatment with small molecules combined with live cell imaging has been shown to be very useful in understanding the dynamic processes of membrane trafficking. Small molecule Endosidin2 (ES2) has been found to target Exo70 protein and inhibit exocytosis and promote vacuolar trafficking in plants. Here we describe the method of using short-term ES2 treatment combined with live cell imaging to study plant exocytosis and vacuolar trafficking in Arabidopsis seedlings expressing a cargo protein PIN2:GFP.
Published Version
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