Abstract
Objective: To evaluate methods for analysis, biological variation, and reference ranges for 6β-hydroxycortisol in urine. Methods: The urinary level of 6β-hydroxycortisol (6β-OHF) was measured by reversed-phase high performance liquid chromatography. Results: The method was accurate and precise, as indicated by a recovery rate of 92–95% and less than 5.5% and 6.6% of coefficient of variation for within-run and between-run assay, respectively. The reference range of urinary 6β-OHF was 187–1245 nmol/day in adults (20–60 years) and 103–915 nmol/day in the elderly (>61 years). No significant day-to-day intrabiological variation was observed in a 5-day period. Diurnal rhythms of urinary 6β-OHF and urinary free cortisol (UFC) were quite parallel to each other, so that the ratio of 6β-OHF to UFC remained constant through the day. Conclusion: An early morning urine specimen can provide the urinary ratio of 6β-OHF to UFC as adequately as that determined from 24-h specimen.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
