Abstract
Artimisia scoparia has been used in the treatment of different disorders including ulcers. The current study was therefore designed to investigate the aerial parts of Artemisia scoparia (crude extract, total sterol and flavonoidal contents, and aqueous fraction) for its urease inhibitory potential. The crude of the plant evoked marked attenuation on urease activity, when tested in various concentrations with IC50 values of 4.06 mg/ml. The inhibitory potential was further augmented in the aqueous fraction (IC50: 2.30 mg/ml) of the plant. When the total sterol and flavonoidal contents were challenged against urease, both showed concentration dependent activity; the latter showed maximum potency with IC50 values of 8.04 and 2.10 mg/ml, respectively. In short, the aerial parts of the plant demonstrated marked antagonism on urease and thus our study validated the traditional use of Artemisia scoparia in the treatment of ulcer.
Highlights
Urease is usually found in different bacteria, fungi, algae, and plants, an enzyme that catalyzes the hydrolysis of urea to ammonia and carbamate, which is the final step of nitrogen metabolism in living organisms [1]
For the determination of total sterol contents, powdered plant material of aerial parts of the A. scoparia was extracted with methanol 3 times and was concentrated
The results of our study showed profound inhibition of urease (Jack Bean) of the extract, total flavonoidal and sterol contents, and aqueous fraction of A. scoparia in a concentration dependent manner
Summary
Urease (urea amidohydrolase) is usually found in different bacteria, fungi, algae, and plants, an enzyme that catalyzes the hydrolysis of urea to ammonia and carbamate, which is the final step of nitrogen metabolism in living organisms [1]. Carbamate rapidly and spontaneously decomposes, yielding a second molecule of ammonia These reactions may cause significant increase in pH and are responsible for negative effects of urease activity in human health and agriculture [2, 3]. Urease is central to H. pylori metabolism and virulence, is necessary for its colonization of the gastric mucosa, and is a potent immunogen that elicits a vigorous immune response. This enzyme is used for taxonomic identification and for diagnosis and followup after treatment and is a vaccine candidate. Keeping in view the antiulcer use of the plant, the current study was designed to investigate urease inhibitory properties of crude methanolic extract, total flavonoidal and sterol contents, and aqueous fraction of aerial parts of the plant in vitro
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