Abstract
Urease activity was found in the fermented liquid of Thiobacillus thiooxidans. It was found that initial concentration of sulfur and urea, and initial pH influenced urease activity in Thiobacillus thiooxidans, and the maximum urease activity was noted after 96h of fermentation with 24g/L initial concentration of sulfur and 0.1g/L of urea, and nature pH 5.82. The experiment found that urease activity showed a steady change which follow along with the growth of Thiobacillus thiooxidans. It shows a possibility to determine the Thiobacillus thiooxidans attached on the sulfide surface.
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