Abstract
Uptake onto and distribution within Escherichia coli and Shizosaccharomyce pombe of three 14C-radiolabelled isothiazolone biocides, benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-Cl- N-methylisothiazolone (CMIT) has been investigated. ‘C-type’ adsorption was observed in all cases at low concentrations of biocide. For E. coli suspensions, saturation of binding sites was indicated by S/L-type adsorption patterns at elevatedbiocide levels. Levels of uptake by the yeast suspension were twice that by E. coli and 4–5 times greater for CMIT than for BIT or MIT. Of the absorbed biocide, greater fractions of CMIT (4%), than MIT or BIT (2%) were associated with the yeast envelop. similar fractions of all three biocides were associated with Gram-negative envelopes (6–8%). Autoradiographs of envelope and cytosolic fractions taken from biocide-terated suspensions failed to demostrated specific target proteins, rather uniform, low level binding was associated with all of the detectable proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.