Abstract
Uptake onto and distribution within Escherichia coli and Shizosaccharomyce pombe of three 14C-radiolabelled isothiazolone biocides, benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-Cl- N-methylisothiazolone (CMIT) has been investigated. ‘C-type’ adsorption was observed in all cases at low concentrations of biocide. For E. coli suspensions, saturation of binding sites was indicated by S/L-type adsorption patterns at elevatedbiocide levels. Levels of uptake by the yeast suspension were twice that by E. coli and 4–5 times greater for CMIT than for BIT or MIT. Of the absorbed biocide, greater fractions of CMIT (4%), than MIT or BIT (2%) were associated with the yeast envelop. similar fractions of all three biocides were associated with Gram-negative envelopes (6–8%). Autoradiographs of envelope and cytosolic fractions taken from biocide-terated suspensions failed to demostrated specific target proteins, rather uniform, low level binding was associated with all of the detectable proteins.
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